At 7 a.m. a mass began at Don Bosco Church, attended by relatives of the victims.
In the homily, Panama Archbishop José Domingo Ulloa José recalled that incidents involving public transport have resulted in a number of deaths and serious injuries since the bus fire.
"Our roads have become paths of self-destruction," he said. This situation is made worse by the poor state of the streets, vehicular congestion, recklessness and the endless hours spent waiting for public transport, the priest said.
"This is a day for national reflection so that we can all shoulder our responsibilities," he said. "The crisis in the transit system deserves special attention."
Julio González, director of the transit agency ATTT, said this day represents a call to society as a whole so that traffic accidents can be reduced. There have been 500 deaths so far this year.
At the same time, the official stressed that the public transport system is a "work in progress" and that significant steps have been taken.